Degradation of the neolignan, burchellin in the hemolymph of the bloodsucking insect Rhodnius prolixus

The neolignan, burchellin, a natural compound that reduces urine excretion in larvae of the bloodsucking bug, Rhodnius prolixus, a vector of Chagas' disease, is rapidly degraded in the hemolymph of the insect. The main product that accumulates in this tissue has been shown to be piperonyl alcohol. Other catabolites have been identified by GC-MS analysis.     

Floristic and structural recovery of a laurel forest community after clear-cutting: A 60 years chronosequence on La Palma (Canary Islands)

We analyzed a post-clearcut chronosequence (0.5 to 60 years after harvesting) in the laurel forest of La Palma island (Canarian Archipelago) to determine the recovery of the stands with respect to species composition, richness, life strategies and structural parameters of the canopy. Multivariate analysis showed that exotic species, as well as annual ruderal species were confined to early-successional stages, while native perennials, typical of laurel forests, dominated the late-successional stages. Total species richness decreased significantly with time after clear-cutting. The relative fast recovery of understory native species may be due to low forest floor disturbance during harvesting. Shade-intolerant pioneer, pioneer-remnant and shade-tolerant late-successional species were the main life strategies of native tree species. Most structural parameters showed a continuous and monotonic increase (basal area, biomass) or decrease (density, percentage of photosynthetic biomass) during succession. Once clear-cutting, here performed with an interval of 8 years, is abandoned, the recovery of the laurel forest seems possible due to careful logging that protects the soil and a rapid asexual regeneration of native tree species, revealing this to be a sustainable management practice.     

Long-term adaptation potential of Central European mountain forests to climate change: a GIS-assisted sensitivity assessment

An ecological risk assessment is described for determining the adaptation potential of the approximately 11 000 Swiss Forest Inventory points (FIP) to a hypothetically changing climate. The core of the study is a spatially explicit forest community model that generates estimates of the potential natural vegetation for the entire potential forest area of Switzerland under today's as well as under altered climate regimes. The model is based on the Bayes formula. The probabilities of the communities occurring along ecological gradients are derived from empirical data featuring the relationships between quasi-natural vegetation types and measured site variables. Bioclimatological input variables are the quotient between July temperature and annual precipitation (model version A) or mean annual temperature (model version B). Other site variables include aspect, acidity of top soil and, to account for continentality, geographical region. Climate change scenarios are defined as follows: ‘Moderate climate change’ implies an increase of the mean annual temperature of 4°C to 1.4°C depending on the region (model version B) or an increase of the July temperature of 1.5°C (model version A). ‘Strong climate change’ implies an increase of the mean annual temperature of 2°C to 2.8°C (model version B) or an increase of the July temperature of 3.0°C (model version A).

The simulation experiment showed that the geographical distribution of 15 potential natural forest types (distinguished on the basis of floristic affinities) varies considerably with changing temperature. Under moderate warming 30–55% of the FIP change their potential natural vegetation type, whereas under strong climate change the values increase to 55–89% depending on the model version used. In the ecological risk assessment the existing tree species composition on any FIP was compared with the expected tree species composition under today's as well as under altered climate regimes. A major finding indicated that, under the current climate conditions, approximately 25–30% (depending on the model version used) of all FIP must be considered as poorly adapted, i.e. less than 20% of the actual basal area consists of tree species that are expected as dominating taxa. This definition applies for trees with a diameter at breast height (DBH) ≥ 12 cm. Moderate warming increases the percentage of poorly adapted FIP by 5–10% (relative to all FIP considered), strong warming leads to a 10–30% increase of poorly adapted FIP (relative to all FIP considered). If trees with a DBH < 12cm are considered, the percentage of FIP that have to be classified as poorly adapted is reduced significantly. There are strong regional differences as exhibited in risk maps of 10 km × 10 km resolution.     

Studies of the mechanism of transgene integration into plant protoplasts: improvement of the transformation rate

Summary The production of transgenic plants by means of direct gene transfer to protoplasts is now a widely-used technique. The biological mechanisms underlying the transformation are still poorly understood, but many investigations have attempted to shed light on some components of this process. Varying the experimental conditions has in some cases led to better transformation rates, but further improvements of the protocols are possible. Such improvements will require a better understanding of how the alien DNA enters the cells, becomes integrated into the chromosomes and is treated as a part of the plant genome. Irradiation with sublethal doses of X-rays or UV-light has been shown to increase the transformation frequency, while certain drugs have been shown to act in a similar manner. The effects of these and other factors are discussed.Abbreviations Aph aphidicolin - ATF absolute transformation frequency - BLM bleomycin - CaMV cauliflower mosaic virus - CAT chloramphenicol acetyl transferase - CHO Chinese hamster ovary cells - EF enhancement factor - Nos nopaline synthase - NPTII neomycin phosphotransferase II - Ocs octopine synthase - PEG polyethyleneglycol - RTF relative transformation frequency     

Towards breeding of triploid chamomile (Matricaria recutita L.) – Ploidy variation within German chamomile of various origins

German chamomile is an important medical plant with a long history of usage and a wide range of medical applications. Wild forms are diploid, whereas cultivated ones are diploid and tetraploid. Ploidy level variation within 15 origins (varieties, accessions, populations) of chamomile was investigated. Both naturally occurring triploids and those induced through directed crosses between diploid and tetraploid parents were identified and analysed, and these data could facilitate the exploitation of triploidy in chamomile as in other crop plants (fruit and ornamental plants).     

Changes in contents of carotenoids and vitamin E during tomato processing

The aim of this study was to investigate the effect of different types of tomato processing on contents of lycopene, beta-carotene, and alpha-tocopherol. Samples of tomato sauce, tomato soup, baked tomato slices, and tomato juice were taken at different times of heating, respectively, after each step of production. HPLC was used to analyze contents of carotenoids and vitamin E. Due to the loss of water during thermal processing, contents of lycopene, beta-carotene, and alpha-tocopherol on a wet weight basis increased. On a dry weight basis, contents of lycopene increased or decreased depending on the origin of the tomatoes used, whereas the beta-carotene contents decreased or were quite stable. In contrast to lycopene, beta-carotene isomerized due to thermal processing. The alpha-tocopherol contents significantly rose during short-term heating. The increase was not caused by release of alpha-tocopherol from the seeds containing predominantly gamma-tocopherol and accounting for 2% of total alpha-tocopherol content only.     

Die klassifizierung von bodenprofilen als grundlage agrarer standortplanung in Entwicklungsländern. Ein beispiel aus dem savannengebiet Nordost-Ghanas

One of the major tasks of experimental farms is the determination of productivity with regard to the regionally differing natural, technological, and socio-economic boundary conditions. In order to achieve this aim the location of the respective stations must be regionally representative from the viewpoint of the major factors controlling agrarian production. In paradigmatic representation, i.e. focusing the very interest on soil qualities and dealing with the north-eastern part of Ghana as a developing country, this article describes a cogent method for defining local representativity of production sites by means of classification procedures and biplot calculus. The application of the latter derivative of matrix calculus appears absolutely necessary for defining those results of clustering procedures which are interpretable in factual terms.     

Marking the success or end of global multi-stakeholder governance? The rise of national sustainability standards in Indonesia and Brazil for palm oil and soy

The RSPO and RTRS are global private partnerships that have been set up by business and civil society actors from the North to curb de-forestation and to promote sustainable production of palm oil or soy in the South. This article is about the launch of new national standards in Indonesia and Brazil that are look-alikes of the global standards but have been set up and supported by government or business actors from the South. The two main questions of this article are: do the new national standards in Indonesia and Brazil provide a fundamental challenge to the RSPO and RTRS, or do they demonstrate the successful diffusion and adoption of global private rules into national contexts? Do the new national standards help or undermine the RSPO and RTRS in their efforts to reduce de-forestation? Combining the theoretical notions of proto-institution and rival governance network, a comparative analysis is offered of the launch of the new national standards in Indonesia and Brazil. The conclusion is that, whilst the RSPO and RTRS have served as models for the general design and principles of the national standards, they really differ from the global standards in terms of normative contents: the national standards offer more room to palm oil plantations and large-scale soy producers to expand production at the expense of forests and other high conservation areas. Governments and producer associations in Indonesia and Brazil have not launched national standards to implement the RSPO or RTRS but to challenge these interventions from the North.